ELISPOT assays for cytokines and protein production measured at single cell level permitting rapid analysis for exceptional high throughput and extraction of detailed information enumerated from large sample numbers.
FluoroSpot assays, permitting directly labeled detection, no enzymatic amplification or substrate, and six-color simultaneous detection permitting fewer cells and reagents.
Fluorescence-based Cell Counting: powerful alternative to flow cytometry being: faster (with up to one million cells in an image analyzed in a couple seconds vs. much longer for the same number of cells by flow), more sensitive for rare cells (detection limit one fluorescent cell per million bystander cells i.e. 0.0001%), more economical (every cell is counted, none lost in system), lends itself to high-throughput versus flow, viability counts (live/dead/apoptotic cell counts), FluoroSpot nuclear counting (to count hyperplasia, tissue infiltration, or cells).
Microbial assays: microbial load and bioburden testing–enabling colony miniaturization and automated analysis, increasing speed, throughput, and reproducibility.
Colony studies permitting automated size-selection and enumeration for clonogenic assays and identifying multipotent progenitor cells.
Genotoxic assays for imaging, measuring, and quantifying mutagenic potential by counting cells (mouse lymphoma assays) or microbes (e.g. Ames test).
Viral plaque assays performing multiple object-oriented morphometric measurements, enabling user-defined gating by imaging and analyzing the monolayer or bacterial lawn.
Quantification of immunohistochemistry and immunofluorescence slides and stains, including multiple-color fluorescent slide images–providing objective quantification along with a recorded image.
Multiplex immunoassays analyzing broad selection of inflammation and immunology biomarkers available in a variety of human and animal models to investigate the modulation and expression of dozens of analytes simultaneously, with advantage of speed and sensitivity, which can improve productivity.
Multiplex enzyme assays investigating cellular metabolites applicable to multiple research areas and diseases, including neurodegeneration, aging, metabolic syndrome, obesity, cancer, cardiovascular disease and toxicity.
Multiplex receptor-ligand assays to obtain quantitative data: including absolute concentrations using synthetic peptides as standards; measurement of key signaling pathways with analytically validated, preconfigured kits; measurement multiple phosphorylation sites on the same protein in the same well, measure both total and phosphorylated protein in the same well.
Multiplex nucleic acid hybridization assay by multiplexing of up to 100 custom-targets in one well using the suspension array system; simple single nucleotide polymorphism (SNP) genotyping using specialty software, specifically designed for integrated allelic discrimination; specialty software analysis for applications in SNP analysis, pathogen detection, strain typing, halotyping, and gene expression.
Multiplex Assays in a variety of Panel Configurations for cytokines, chemokines, growth factors, TGF-b, the Th17 pathway, cell signaling, Cancer, Diabetes, Isotyping, Acute phase, custom designed multiplex bead assays.
Multiplex Protein Quantitation to simultaneously examine multiple phosphorylation events involving upstream or downstream molecules in one or more cellular signaling pathways, maximizing information obtained from a single sample by permitting the analysis of more than one target protein at a time, with minimal sample volume requirements for evaluating the phosphorylation states of specific phosphoproteins or the expression levels of total target proteins.
Multiplex Gene Expression to quantitatively measure multiple RNA targets simultaneously with high accuracy and precision, quantitation of original RNA population directly from lysates avoids biases, sample loss, false positive/negative results associated with RNA isolation, cDNA synthesis, PCR amplification. High accuracy and precision using branch DNA (bDNA) signal amplification versus target amplification for multiplexing 3 to 80 target RNAs and precision results in difficult clinical research samples, including, H&E-stained FFPE, blood, and skin.
Multiplex Genotyping (SNP) – low to medium throughput genotyping projects, capable of analyzing anywhere from one SNP in one subject or sample or up to 50 SNPs in unlimited numbers of subject. The fluorescent microspheres used in the SNP assay are coupled to oligonucleotides (a “TAG sequence”) such that each bead’s fluorescent address is associated with a known nucleotide sequence. User-provided oligos, comprised of a SNP specific sequence coupled to the complement of the bead-associated oligo sequence, are used in an allele specific extension reaction with biotinylated nucleotides. These products are combined with selected fluorescent bead-oligo combinations. Reaction products are then analyzed on the Bio-Plex instrument, which quantifies the amount of PE signal associated with a particular fluorescent bead address. Each address is linked to a particular oligo which in turn is linked to a particular SNP based on the experimental design. Thus, differences in signal intensity determine which alleles are present in a given input PCR fragment
Custom Qualitative Ratio Metric Assays: Any experimental design that can be successfully bound to a Fluorescent bead can be used as a direct qualitative ratio metric to identify unique analytes of interest in many varieties of suspensions.